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Fig. 7 | Journal of Neuroinflammation

Fig. 7

From: Pellino1 regulates neuropathic pain as well as microglial activation through the regulation of MAPK/NF-κB signaling in the spinal cord

Fig. 7

LPS-induced inflammatory reactions were suppressed by inhibition of Peli1 in BV2 microglial cells. a, b BV2 cells were pretreated without or with 10, 100, and 1000 ng/ml LPS for 2 h. Peli1 mRNA (a) and protein (b) levels were determined by qPCR and western blot respectively. One-way ANOVA [(a) F3,14 = 11; (b) F3,11 = 7.9]. c–f BV2 microglial cells were transfected with Peli1 siRNA or negative siRNA for 32 h before stimulated without or with LPS (100 ng/ml) for 2 h. Transfection of Peli1 siRNA suppresses LPS-induced increases in Peli1 mRNA (c) and protein (d) levels. One-way ANOVA [(c) F3,13 = 26.9; (d) F3,17 = 13.8]. (e) ELISA analysis showing TNF-α, IL-1β, and IL-6 release in BV2 microglia culture medium and the inhibitory effects of Peli1 siRNA on the release. One-way ANOVA (F2,9 = 134.8; F2,8 = 8.5; F2,11 = 35.1). (f) Western blot showing the inhibitory effects of Peli1 siRNA on the changes of phosphorylation level of MAPK and NF-κB p65 in cell lysates of BV2 cells were subjected to LPS stimulation. Results are expressed as the Mean ± SEM. *p < 0.05, **p < 0.01, ***p < 0.001 compared with indicated group

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