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Fig. 8 | Journal of Neuroinflammation

Fig. 8

From: Pellino1 regulates neuropathic pain as well as microglial activation through the regulation of MAPK/NF-κB signaling in the spinal cord

Fig. 8

LPS-induced BV2 microglial activation was suppressed by inhibition of Peli1. a After LPS stimulation, transfected BV2 cells were fixed and labeled with DAPI (blue) and anti-Iba1 (red, scale bar 50 μm). b qPCR showing the inhibitory effects of Peli1 siRNA on the mRNA expression of Iba1 in BV2 cells after LPS treatment. One-way ANOVA (F2,14 = 5.9). c The effects of Peli1 on microglial M1 polarization markers (TNF-α, IL-1β, IL-6, CD86, iNOS) and M2 polarization markers (Arg1, Ym1, CD206, IL-10) mRNA expression in BV2 cells after LPS treatment were revealed by qPCR. One-way ANOVA (F2,10 = 10.9; F2,9 = 7.3; F2,10 = 15.2; F2,12 = 6.2; F2,12 = 67.0; F2,9 = 16.2; F2,9 = 8.4; F2,9 = 1.2; F2,9 = 6.6). d After 24 h of transfection, scratch assay in BV2 cells treated with 100 ng/ml LPS for 24 h. Transfection of Peli1 siRNA inhibits BV2 cells migration into the wound area. Representative images were taken before and 24 h after injury (× 4 magnification). Dashed line indicates the width of gap. Results are expressed as the Mean ± SEM. *p < 0.05, **p < 0.01, ***p < 0.001 compared with indicated group

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