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Fig. 1 | Journal of Neuroinflammation

Fig. 1

From: Pathological modeling of TBEV infection reveals differential innate immune responses in human neurons and astrocytes that correlate with their susceptibility to infection

Fig. 1

TBEV infects, replicates, and spreads in hNPC-derived brain cells. a Schematic representation of the experimental procedure. b Immunofluorescence labeling of differentiated hNPCs 24 h and 72 h following TBEV infection. An antibody directed against the domain 3 of the viral envelope (TBEV-E3, green) revealed infected cells. Nuclei were stained with DAPI (blue). c Enumeration of infected cells based on immunofluorescence labeling using an ArrayScan Cellomics instrument. d RNA from the supernatant and cell lysate of infected cells was analyzed by RT-qPCR to determine viral replication. e Supernatant was collected at 14, 48, and 72 hpi and titrated by endpoint dilution (TCID50) on VERO cells. Results are representative of 3 independent experiments performed in triplicate. Data are expressed as the mean ± SD. Statistical analysis was performed using one-way ANOVA (Bonferroni’s Multiple Comparison Test) with Graphpad Prism V6.0.1. ns, non-significant (p > 0.05); **p < 0.01, ***p < 0.001. Scale bar = 100 μm

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Journal of Neuroinflammation

ISSN: 1742-2094