Fig. 5
TBEV-induced antiviral response in hNPC-derived neuronal/glial cells. a TBEV-infected neuronal/glial cells (MOI 10−2) and their matched NI controls were analyzed 24 hpi using an RT2 Profiler PCR array specific for the human antiviral response. The heat map shows the differential expression of 84 analyzed human genes. The most highly up- and downregulated genes are colored in red and dark green, respectively. The blue lines indicate the arbitrary cut-off of 3. Genes between the two lines are considered nonregulated. b–k RT-qPCR analyses of selected antiviral genes. Gene expression was normalized to HPRT1 gene, and the − 2ΔΔCt method was used for relative quantification (normalization to non-infected cells at 7 hpi). Data are expressed as the mean ± SD. Results are representative of one experiment performed on pooled triplicates (PCR array) or two independent experiments performed in triplicate (qPCRs). Statistical analysis was performed using a two-tailed unpaired t test with Graphpad Prism V6.0.1. ns, non-significant (p > 0.05); *p < 0.05, **p < 0.01, ***p < 0.001