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Fig. 6 | Journal of Neuroinflammation

Fig. 6

From: Pathological modeling of TBEV infection reveals differential innate immune responses in human neurons and astrocytes that correlate with their susceptibility to infection

Fig. 6

Enrichment of human neurons and astrocytes by magnetic-activated cell sorting. HNPC-derived neuronal/glial cells differentiated for 13 days were sorted using MACS technology. a, e, h Phase-contrast micrographs, showing Uns-C (a), En-N (e), and En-As (h), were acquired 96 h after re-seeding. Blue arrows indicate neurons and brown arrowheads indicate astrocytes. Scale bars, 50 μm. b, f, i Immunofluorescence staining with antibodies directed against neurons (HuC/HuD, green), astrocytes (GFAP, red), and oligodendrocytes (OLIG2, gray) markers showing the mixed and enriched population. DAPI is in blue. Scale bars = 100 μm. c, g, j Enumeration of neurons (Ne), astrocytes (As), and oligodendrocytes (Ol) in Uns-C (c), En-N (g), and En-As (j) based on immunofluorescence staining and using an ArrayScan Cellomics instrument. Data are representative of 4 independent experiments performed in triplicate. d Scatterplot of basal level of antiviral response genes in unsorted cells (Uns-C) compared with non-trypsinized cells (Co-C). Analysis was performed using an antiviral response PCR array. Genes along the black line have similar expression levels in the two cultures. Dotted lines represent an arbitrary cut-off of 3. Data are from a single experiment performed with pooled triplicates

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