Fig. 7
Antiviral response in enriched neurons, enriched astrocytes, and unsorted cells. a–i Expression of antiviral genes upon TBEV infection. a TBEV-infected Uns-C, En-N, and En-As (MOI 10−2) and their matched non-infected controls were analyzed 24 hpi using an RT2 profiler PCR array specific for the human antiviral response. Heat map showing the 84 human genes analyzed and their differential expression. Color code and blue line are as in Fig. 5. Note that genes noted (+) are above the cut-off of 3. b–k RT-qPCR analyses of selected antiviral response genes in En-As (red) and En-N (green). l–p Basal expression of antiviral genes. l, m Scatterplots of basal expression levels of antiviral response genes in enriched neurons (En-N) compared with that of unsorted cells (Uns-C) (l, k) or enriched astrocytes (En-As) (m). Analysis was performed using an antiviral response PCR array. n–p RT-qPCR analysis of the basal expression of IFIH1/MDA5 (n), DDX58/RIG-I (o), and OAS2 (p) genes in En-N and En-As. Gene expression was normalized to HPRT1 and the − 2ΔΔCt method was used for relative quantification (normalization to non-infected En-N, at 7hpi for b–k). The results are expressed as the mean ± SD. Data are representative of two independent experiments performed in triplicate (b–k, n–p) and one experiment performed with pooled triplicates (l, m). Statistical analyses comparing En-As and En-N were performed with Graphpad Prism V6.0.1 using a two-tailed unpaired t test. ns, non-significant (p > 0.05); *p < 0.05, **p < 0.01, ***p < 0.001