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Fig. 1 | Journal of Neuroinflammation

Fig. 1

From: Mutant Huntingtin affects toll-like receptor 4 intracellular trafficking and cytokine production in mast cells

Fig. 1

Characterization of WT and R6/1 BMMCs. Bone marrow-derived mast cells from WT or R6/1 mice were cultured as described in the Methods section. a Representative electronic scanning micrograph of WT and R6/1 BMMCs (10,000×). b Mean diameter of WT- and R6/1 BMMCs. c Representative electronic transmission micrograph of WT and R6/1 BMMCs. d Number of granules per cell in WT and R6/1 BMMCs. e, f Expression of FcεRI or TLR-4 receptors on the plasma membrane of WT and R6/1 BMMCs as determined by flow cytometry. g RT-PCR showing the expression of TLR-4, MD-2, and CD14 mRNAs in WT and R6/1 BMMCs. Analyses shown on (b) were performed by measuring 27–32 cells from at least three different cultures; analyses shown on (d) were performed by measuring 15–20 cells from at least three different cultures of each genotype by transmission electron microscopy. Data are presented as mean ± SD. Panels e, f show a representative flow cytometry plot from at least three experiments performed with different cell cultures. The image shown on panel g is representative of two experiments performed with different cell cultures

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