Fig. 2

FcεRI-induced β-hexosaminidase release and TNF production after TLR-4 receptor activation in R6/1 BMMCs. a Dose-response curve of β-hexosaminidase release by BMMCs. b Time-course of β-hexosaminidase secretion by BMMCs after DNP-HSA (27 ng/mL) treatment. c Time-course of TNF secretion after LPS (500 ng/mL) treatment to WT and R6/1 BMMCs. TNF in cell supernatants was quantified by ELISA. d Time-course of TNF mRNA expression in WT and R6/1 cells after LPS treatment (500 ng/mL). Left panel shows a representative experiment; right panel shows densitometric analysis of different experiments. Data are presented as the mean ± SD of at least three independent experiments performed with different cell cultures. *p < 0.05, *** p < 0.001 vs. WT cells; ^&&p < 0.01, ^&&&p < 0.001 vs. the value at time zero or in non-stimulated cells, as determined by two-way ANOVA followed by Tukey’s test