Fig. 6

Impaired LPS-induced TLR-4 and Htt vesicular trafficking in R6/1 BMMCs. The cells were stimulated with LPS (500 ng/mL) and then processed for confocal microscopy to detect TLR-4 and Htt at 0, 15, 30, and 60 min after stimulus. a Representative images (n = 60–70 cells per condition) are shown. The images were taken at a × 63 magnification. Scale bar = 2 μm. TLR-4 is shown in red, Htt is shown in green, and cell nuclei are shown in blue. b Percentage of cells with a perinuclear localization of TLR-4. c Percentage of cells showing a perinuclear localization of Htt. Pearson’s correlation coefficient for TLR-4 and Htt in d the whole area and in e the perinuclear area of the images obtained from the equatorial slices. Data are presented as the mean ± SD (n = 4). ^&p < 0.05 vs. the value at time zero or in non-stimulated cells as determined by two-way ANOVA and post hoc Tukey’s test