Fig. 8

Microbiota ablation with antibiotics eliminated MAC supplementation in improving colon length, endotoxemia, and cognition of diet-induced obese mice. a Levels of feces bacterial DNA were quantitated by qPCR after chronic antibiotic treatment (n = 6). b The quantification of colon length was statistically analyzed (n = 9). c Serum LPS level (n = 10). d Nest score (n = 12–15). e Untorn nesting material. f The discrimination ratio. g Total object exploration time in temporal order memory test. Values are mean ± SEM. *p < 0.05 vs. high-fat and fiber-deficient (HF-FD). #p < 0.05 vs. HF-MAC