Fig. 4

ES and APP/PS1 overexpression affect GFAP coverage in the HPC of 10-month-old mice. Representative images of the HPC of 10-month-old WT-CTL (a), WT-ES (b), APP/PS1-CTL (c), and APP/PS1-ES (d) mice. ES modulates the effect of APP/PS1 on GFAP expression in the HPC (e). GFAP coverage in the CA was decreased by both ES and APP/PS1 (f). APP/PS1 increases hippocampal GFAP expression while ES decreases GFAP expression in the SLM (g). GFAP+ cell complexity was unaffected by either ES or APP/PS1 (h). Representative images of clustered GFAP coverage in the HPC of 10-month-old WT-CTL (i), WT-ES (j), APP/PS1-CTL (k), and APP/PS1-ES (l) mice. Clustered GFAP coverage was increased by APP/PS1 overexpression but decreased by ES in whole HPC (m), CA (n), and SLM (o) region. Statistical analyses were performed using two-way ANOVA. ^$Significant effect of genotype p < 0.05; ^*Significant effect of condition p < 0.05; ^{^}Significant post hoc effect p < 0.05; scale bars 250 μm