Vitamin B12 is neuroprotective in experimental pneumococcal meningitis through modulation of hippocampal DNA methylation

Table 1 Effects of BM and adjuvant therapy with vitamin B₁₂ on sulfur amino acids and their metabolites in the hippocampus

	Concentrations in groups (μmol/g of protein)				P values (2-way ANOVA)
	Sh-Sal	Sh-B₁₂	Infect-Sal	Infect-B₁₂	Effect of BM	Effect of vitamin B₁₂	Interaction
Hcy	0.34 ± 0.13	0.33 ± 0.08	0.79 ± 0.49	0.74 ± 0.38	0.0053	0.8286	0.9079
Cys	1.66 ± 0.63	1.8 ± 0.46	2.85 ± 1.4	2.13 ± 0.67	0.0530	0.4447	0.2623
Total GSH	4.94 ± 1.47	5.41 ± 0.46	4.13 ± 1.41	3.29 ± 1.15*	0.0041	0.6975	0.1698
Reduced GSH	3.72 ± 0.97	3.98 ± 0.52	2.98 ± 0.94	2.57 ± 1.05	0.0052	0.8268	0.3451

Data are expressed as means ± S.D. Statistical differences were assessed using a 2-way ANOVA (Bonferroni post hoc test) to examine the effects of meningitis and adjuvant therapy with vitamin B₁₂.
*Statistically significant differences (P < 0.05) at Bonferroni post hoc test when compared with the respective sham-infected control (Sh-Sal or Sh-B₁₂)
Sh-Sal sham-infected + saline, Sh-B₁₂ sham-infected + vitamin B₁₂, Infect-Sal infected + saline, Infect-B₁₂ infected + vitamin, B₁₂Hcy homocysteine, Cys cysteine, GSH glutathione

ISSN: 1742-2094