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Fig. 3 | Journal of Neuroinflammation

Fig. 3

From: DJ-1 exerts anti-inflammatory effects and regulates NLRX1-TRAF6 via SHP-1 in stroke

Fig. 3

DJ-1 regulated the expression of NLRX1, TRAF6, and SHP-1 after cerebral I/R injury. a, c Western blot detecting NLRX1, TRAF6, and SHP-1 in rats. b, d Western blot detecting NLRX1, TRAF6, and SHP-1 in astrocytes. e Immunohistochemistry was used to measure NLRX1 expression in mitochondria in rats after DJ-1 knockdown. Original magnification, × 400. f Immunocytochemistry was used to measure NLRX1 expression in mitochondria in astrocytes. Original magnification, × 600. Fluorescence microscopy was used to assess NLRX1 expression, which is indicated by red fluorescence. HSP60 expression is indicated by green fluorescence. Cell nuclei were stained with DAPI. The data are expressed as the mean ± SEM. *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001. n = 6 per group

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