Fig. 5

DJ-1 inhibited the expression of TNF-α, IL-1β, and IL-6 after cerebral I/R injury via SHP-1. a, c After virus and TPI-1 were used to overexpress DJ-1 and inhibit SHP-1, respectively, Western blotting was used to detect the cytokines IL-1β, IL-6, and TNF-α in rats. b, d After virus and TPI-1 were used to overexpress DJ-1 and inhibit SHP-1, respectively, Western blotting was used to detect the cytokines IL-1β, IL-6, and TNF-α in astrocytes. e, g After treatment with an SHP-1 inhibitor, Western blotting was used to detect the cytokines IL-1β, IL-6, and TNF-α in rats. f, h After treatment with an SHP-1 inhibitor, Western blotting was used to detect the cytokines IL-1β, IL-6, and TNF-α in astrocytes. i, k, m Quantification of TNF-α, IL-1β, and IL-6 in rats by ELISA. j, l, n Quantification of TNF-α, IL-1β, and IL-6 in astrocytes by ELISA. The data are expressed as the mean ± SEM. *p < 0.05 vs. the sham group; ^#p < 0.05 vs. the MCAO group; ^&p < 0.05 vs. the overexpression group; ^★p < 0.05 vs. the DMSO group; ^△p < 0.05 vs. the DMSO group; ^◆p < 0.05 vs. the TPI-1 group. n = 6 per group. The data are expressed as the mean ± SEM. *p < 0.05 vs. the control group; ^#p < 0.05 vs. the OGD/R group; ^&p < 0.05 vs. the overexpression group; ^★p < 0.05 vs. the DMSO group; ^△p < 0.05 vs. the DMSO group; ^◆p < 0.05 vs. the TPI-1 group. n = 6 per group. The groups in a and b and their corresponding groups in the statistical graphs are as follows: sham (−−−), MCAO or OGD/R (+−−), scramble (+−−), overexpression (++−), overexpression + DMSO (++−), and overexpression + TPI-1 (+++). The groups in e and f and the corresponding groups in the statistical graphs are as follows: DMSO (+−−), TPI-1 (+−+), overexpression + DMSO (++−), overexpression + TPI-1 (+++)