Fig. 6

DJ-1 regulated the disassociation of NLRX1 from TRAF6 after cerebral I/R injury via SHP-1. a, g After virus and TPI-1 were used to overexpress DJ-1 and inhibit SHP-1, respectively, Western blotting was used to detect NLRX1, TRAF6, and SHP-1 in rats. b, h After virus and TPI-1 were used to overexpress DJ-1 and inhibit SHP-1, respectively, Western blotting was used to detect NLRX1, TRAF6, and SHP-1 in astrocytes. c, i After treatment with an SHP-1 inhibitor, Western blotting was used to detect the cytokines IL-1β, IL-6, and TNF-α in rats. d, j After treatment with an SHP-1 inhibitor, Western blotting was used to detect the cytokines IL-1β, IL-6, and TNF-α in astrocytes. e Immunoprecipitation and immunoblot analyses of NLRX1-TRAF6 in rats. f Immunoprecipitation and immunoblot analyses of SHP-1-TRAF6 in rats. The data are expressed as the mean ± SEM. *p < 0.05 vs. the sham group; ^#p < 0.05 vs. the MCAO group; ^&p < 0.05 vs. the overexpression group; ^★p < 0.05 vs. the DMSO group; ^△p < 0.05 vs. the DMSO group. n = 6 per group. The data are expressed as the mean ± SEM. *p < 0.05 vs. the control group; ^#p < 0.05 vs. the OGD/R group; ^&p < 0.05 vs. the overexpression group; ^★p < 0.05 vs. the DMSO group; ^△p < 0.05 vs. the DMSO group. n = 6 per group