Fig. 2

Flow chart illustrating two experimental paradigms. a Single application. The catheterization was performed 4 days prior to SNI operation. LA at 3, 10, 30 μM was i.t. applied on day 5 post nerve injury. Mechanical and thermal tests were conducted 1 day prior to nerve injury and on POD 5 and 6. After drug application, mechanical tests were elaborately performed six times within 3 h, i.e., every 30 min a time. The cold allodynia was observed every 1 h, totally three times. b Multiple applications. The operation schedule for catheterization and SNI was the same as the single paradigm. LA at 30 μM, TRPC6 antisense or mismatch ODNs with or without supplementation of LA at 30 μM was i.t. applied daily from day 1 to 6 after SNI. Behavioral tests were performed pre- and post-drug application on day 5. On day 6, the drug was applied again and rats were sacrificed around 2.5 h post application after the final behavioral observation. Tissue collections for immunohistochemical (IHC) staining, Western blot (WB) and multiplex measurement (ELISA) were performed thereafter