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Fig. 3 | Journal of Neuroinflammation

Fig. 3

From: The analgesic action of larixyl acetate, a potent TRPC6 inhibitor, in rat neuropathic pain model induced by spared nerve injury

Fig. 3

The analgesic action of LA after single or multiple applications. a Single application of LA at 3, 10, and 30 μM on POD 5 dose-dependently alleviated mechanical allodynia. The effect of LA at 10 μM in rats with SNI was transiently observed at 2 h after i.t. application. The action of LA at 30 μM lasted longer and was maintained to 3 h. Vehicle (0.3% chloroform) or 30 μM of LA had no marked influences on mechanical response of sham-operated rats. b Mechanical test on POD 5 before (“pre”) and after the fifth application of LA at 30 μM. Multiple applications not only enhanced the analgesic efficacy but also elongated action duration. The data for SNI + single (sin) group were adopted from (a). c Cold sensitivity on POD 5 before and after application of acetone. Acute i.t. administration of LA at 30 μM gradually relieved cold allodynia induced by SNI but did not cause visible effects on sham animals. d Multiple applications of LA at 30 μM advanced the onset time to 1 h post drug but did not significantly elongated the action duration to the second day. The data for SNI + single group were adopted from (c). eg Open field test showing that multiple applications of LA in catheterized normal rats did not cause marked influences on animal locomotive activities. e Comparison of the total travel distance in 5 min between vehicle and LA (30 μM) treated groups. f Time spent in central arena (25% area) of the open field. g Typical locomotive traces in two groups. *p < 0.05, **p < 0.01, ***p < 0.001 vs. SNI + veh group. #p < 0.05, ##p < 0.01, ###p < 0.001 vs. SNI + sin group. n = 6–8

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