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Fig. 9 | Journal of Neuroinflammation

Fig. 9

From: The analgesic action of larixyl acetate, a potent TRPC6 inhibitor, in rat neuropathic pain model induced by spared nerve injury

Fig. 9

The changes of pain behaviors and p38 signaling after knockdown of TRPC6. a von Frey filament tests showing that i.t. application of TRPC6 antisense (AS) for 6 days significantly relieved mechanical hypersensitivity in SNI rats compared with mismatch (MM) treatment group. Supplementation with 30 μM of LA rescued the effect of sole mismatch while had no obvious influences on the effect of sole antisense. n = 6–8. b Acetone test showing that treatment with TRPC6 antisense also remarkably suppressed cold allodynia compared with mismatch group. This effect was almost equal to that of the co-administration groups. n = 6–9. c Western blot analysis demonstrated that application of antisense ODN caused significant decrease in the level of TRPC6, the efficacy of which was comparable to the other two groups. n = 5. d Typical picture of TRPC6 immunofluorescent staining illustrating the generally decreased intensity of TRPC6 IR after antisense treatment. CC central canal. Scale bar, 200 μm. e, f Western blot analysis demonstrated that phosphorylated p38 (e) other than p38 (f) was significantly suppressed in the antisense and the two co-administration groups. *p < 0.05, **p <0.01, ***p < 0.001 vs. mismatch group, n = 5

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