Fig. 3

DMI suppresses MG activation in EAE. a C57BL/6 mice (n = 8/group) subjected to EAE were administered with vehicle or DMI (400 mg/kg) every day starting from day 3 post-immunization. At day 12–14 post-immunization, mononuclear cells were isolated from the brain and spinal cord of vehicle- and DMI-treated EAE mice. The isolated cells were then subjected to staining of CD45 and CD11b with CD80 or CD86 followed by FACS analysis. MG were determined based on their expression of CD45^intCD11b⁺, and the surface expression of CD80 and CD86 on CD45^intCD11b⁺ cells was then determined. Isotype controls (ISO) were used as a negative control to determine MG positive for CD80 or CD86 expression. b At day 12 post-immunization, the lumbar region of spinal cord tissues harvested from vehicle- and DMI-treated EAE mice (n = 5/group) was subjected to immunofluorescence analysis of IBA1 expression. The number of IBA1⁺ cells was calculated and quantified. Scale bars, top 200 μm and bottom 100 μm. Statistical significance was determined as ***p < 0.001 and N.S., no significant difference by unpaired t test