Fig. 5

DMI represses the CNS infiltration of encephalitogenic Th1 and Th17 cells in EAE. C57BL/6 mice (n = 8/group) were subjected to EAE induction followed by i.p. administration with vehicle or DMI (400 mg/kg) every day starting from day 3 post-immunization. At day 12–14 post-immunization, mononuclear cells were isolated from the brain and spinal cord of vehicle- and DMI-treated EAE mice. The number of (a) CD4⁺ T cells, (b) IFNγ-expressing CD4⁺ T cells, and (c) IL-17-expressing CD4⁺ T cells in the brain and spinal cord of vehicle- and DMI-treated EAE mice was determined by FACS analysis. ISO were used as a negative control to determine cells positive for the surface expression of CD4 or CD4⁺ cells positive for the intracellular expression of IFNγ or IL-17. Statistical significance was determined as *p < 0.05, **p < 0.01, and ***p < 0.001 by unpaired t test