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Fig. 6 | Journal of Neuroinflammation

Fig. 6

From: Dimethyl itaconate, an itaconate derivative, exhibits immunomodulatory effects on neuroinflammation in experimental autoimmune encephalomyelitis

Fig. 6

DMI suppresses Th1 and Th17 differentiation in vivo and in vitro. Cells were harvested from the spleen of vehicle- and DMI (400 mg/kg)-treated EAE mice (n = 7/group) at day 10 post-immunization. a IFNγ- and IL-17-expressing CD4+ cells and b Foxp3-expressing CD4+CD25+ cells were determined by FACS analysis. ISO were used as a negative control to determine CD4+ cells positive for the intracellular expression of IFNγ or IL-17 or CD4+CD25+ cells positive for the nuclear expression of Foxp3. Statistical significance was determined as *p < 0.05. N.S., no significant difference by unpaired t test. c Naive splenocytes were harvested and polarized into Th1 or Th17 condition in the absence or presence of DMI (150 μM). Forty-eight hours later, cells were harvested and subjected to FACS analysis for the intracellular expression of IFNγ or IL-17 in CD4+ T cells. ISO were used as a negative control to determine CD4+ cells positive for the intracellular expression of IFNγ or IL-17. The representative data of three independent experiments are shown

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