Fig. 4

Immunohistochemical assessment of vessels covered by astrocytes in the cerebral parietal cortex and in the CA1 region of hippocampus. Representative confocal images of astrocyte coverage (GFAP, green) in vessels of the cerebral cortex and hippocampus (IB4, red) of animals which were exercised or sedentary for 12 weeks after cerebral hypoperfusion (2VO-Ex and 2VO-Sed, respectively) or after sham surgery (Sham-Ex and Sham-Sed, respectively). DAPI-labeled nuclei, blue. Quantification of the a cerebral cortex and b hippocampus. Data represent the mean ± SEM of up to 10 vessels per image of 3-4 animals per group. *p < 0.05 vs. Sham-Sed and ^#p < 0.05 vs. 2VO-Sed (ANOVA). Scale bar 50 μm, magnification 200X