Fig. 1

Diagram depicting representative gating strategies for delineating CD4+ T cell subset populations. Following gating for singlets and debris exclusion, the CD4+ T cell population was selected based on CD4 and TCRβ expression. Within this population, the Th1 subset was defined as TBET⁺IFNγ⁺, Th2 as GATA3⁺IL-4⁺, Th17 as RORγt⁺IL-17A⁺, and regulatory T (Treg) helper subset as CD25^highFOXP3⁺