Fig. 4

IHC of CD4+ T cells (green) and IBA+ microglia (red) in the FMNuc. Nissl-labeled neurons are shown in blue. a Composite image of control FMNuc at 14 dpo showing no infiltrating T cells or reactive IBA+ microglia. b Composite image of axotomized FMNuc at 14 dpo. c, d Magnified fields from boxes in b showing IBA1+ microglial nodules (arrowheads) with associated CD4+ T cells. e–h Representative fields from axotomized FMNuc in other experimental animals showing IBA1+ microglial nodules (arrowheads) with associated CD4+ T cells. Scale bar in b = 100 μm and applies to a and b. Scale bar in h = 50 μm and applies to c–h