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Table 3 Antibodies utilized for flow cytometry

From: CD4+ T cell expression of the IL-10 receptor is necessary for facial motoneuron survival after axotomy

Antibody

Manufacturer and cat. no.

Dilution

APC anti-mouse TCR β chain

BioLegend 109212

1:50

Alexa Fluor 488 anti-mouse CD4

BioLegend 100423

1:800

BV 421 anti-mouse CD4

BioLegend 100437

1:50

BV 711 anti-mouse IL-4

BioLegend 504133

1:50

BV 421 anti-mouse IFN-γ

BioLegend 505829

1:50

BV 711 anti-mouse IL-17A

BioLegend 506941

1:50

BV 711 anti-mouse CD25

BioLegend 102049

1:50

PE anti-GATA3

BioLegend 653803

1:50

PE/Dazzleâ„¢ 594 anti-T-bet

BioLegend 644827

1:50

PE anti-RORγt

Invitrogen AFKJS-9

1:50

Alexa Fluor 488 anti-mouse/rat/human FOXP3

BioLegend 320011

1:100

Purified anti-mouse CD16/32

BioLegend 101310

1:1000