Antibody | Manufacturer and cat. no. | Dilution |
---|---|---|
APC anti-mouse TCR β chain | BioLegend 109212 | 1:50 |
Alexa Fluor 488 anti-mouse CD4 | BioLegend 100423 | 1:800 |
BV 421 anti-mouse CD4 | BioLegend 100437 | 1:50 |
BV 711 anti-mouse IL-4 | BioLegend 504133 | 1:50 |
BV 421 anti-mouse IFN-γ | BioLegend 505829 | 1:50 |
BV 711 anti-mouse IL-17A | BioLegend 506941 | 1:50 |
BV 711 anti-mouse CD25 | BioLegend 102049 | 1:50 |
PE anti-GATA3 | BioLegend 653803 | 1:50 |
PE/Dazzleâ„¢ 594 anti-T-bet | BioLegend 644827 | 1:50 |
PE anti-RORγt | Invitrogen AFKJS-9 | 1:50 |
Alexa Fluor 488 anti-mouse/rat/human FOXP3 | BioLegend 320011 | 1:100 |
Purified anti-mouse CD16/32 | BioLegend 101310 | 1:1000 |