Fig. 1

Isolation and purification of microglia and astrocytes. a Schematic showing experimental strategy for isolation and sorting of microglia and astrocytes. b and c RNA-seq analysis of expression of classic cell-specific markers of microglia, astrocytes, neurons, endothelial cells (Endo), oligodendrocyte precursor cells (OPC), and oligodendrocytes (Oligo) in purified microglia (b) and astrocytes (c), as compared with input (total mRNA). Three biological replicates of each time point (microglia and astrocytes) and 2 biological replicates of input are shown