Fig. 7
From: Transcriptomic profiling of microglia and astrocytes throughout aging
Validation of aging-induced changes in microglial and astrocyte gene expression by using in situ hybridization. a–d Bar graphs of RNA-seq data showing FPKM values determined for Cxcl10 in microglia (a), Cxcl10 in astrocytes (b), Ptbp1 in microglia (c), and Ptbp1 in astrocytes (d) upon aging. Columns represent means ± SEM; ****p < 0.0001, ***p < 0.001, **p < 0.01, *p < 0.05, for comparisons of DESeq2-analysis values between 2-month and aging samples (4 months, 6 months, 9 months, 12 months). e and f Representative in situ hybridization images for Cxcl10 (e) and Ptbp1 (f) showing colocalization with a microglial marker (Itgam) and astrocyte marker (Slc1a3) in cortex and hippocampus in 2-month, 4-month, 6-month, 9-month, and 12-month mice. Scale bar, 20 μm. g–j Bar graphs depicting quantification of H-score of Itgam + microglia and Slc1a3 + astrocytes expressing detectable levels of Cxcl10 and Ptbp1 mRNAs upon aging: (g) Cxcl10 in microglia, (h) Cxcl10 in astrocytes (i), Ptbp1 in microglia, and (j) Ptbp1 in astrocytes. One-way ANOVA followed by Dunnett post hoc test; data are shown as means ± SEM; ****p < 0.0001, ***p < 0.001, **p < 0.01, *p < 0.05; n = 3 animals