Fig. 4
From: Astrocytes have the capacity to act as antigen-presenting cells in the Parkinson’s disease brain
Intracellular deposits of αSYN in astrocytes are surrounded by MHC-II. Human astrocytes were exposed to αSYN-F for 7 days and stained for MHC-II and MHC-I. In the presence of IFNγ, the astrocytes clearly upregulate MHC-II (a) and MHC-I (b). The MHC-II totally enclosed the αSYN inclusions, which was confirmed with confocal microscopy (c). Close-up of white rectangle in c is shown in c’. Immunostaining for MHC-I analyzed with confocal microscopy showed a partial encampsulation of the αSYN deposits by MHC-I (d). Close-up of white rectangle in d is shown in d’. Scale bars: a and b = 20 μm, c and d = 5 μm