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Table 1 Primers and parameters of RT-qPCR

From: The impact of hyperpolarization-activated cyclic nucleotide-gated (HCN) and voltage-gated potassium KCNQ/Kv7 channels on primary microglia function

RNA

Sequences forward/reversed 5`-3`

Temperature (°C) step 1/2/3

Duration (s) step 1/2/3

Accession number

Iba1

CCAGCGTCTGAGGAGCTATG/

CGTCTTGAAGGCCTCCAGTT

95/60/72

15/15/45

NM_017196.3

P2RY12

TTGCACGGATTCCCTACACC/

GGGTGCTCTCCTTCACGTAG

95/60/72

15/15/45

NM_022800.1

KCNK13

GAGAACGAAGGGCTCTACCG/

GAAGCACCCGCTATCCAGTT

95/60/72

15/15/45

NM_022293.1

HCN1

CAGAGCACTTCGGATCGTGA/

GGAGCAGCATCATGCCAATG

95/59.8/72

15/15/45

NM_053375.1

HCN2 N° 1

AGATCATCCTGGACCCCGAA/

GGATCTTGGTGAAACGCACG

95/59.8/72

15/15/45

NM_053684.1

HCN2 N° 2

ACCGGCATTGTTATTGAGGAC/

CCTCGGAGTCGATTCCCTTC

95/59.8/72

15/15/45

NM_053684.1

HCN3

GTGCAGTGGTTCGCATCTTC/

GGGAAGTCCTGCAGCATAGG

95/59.8/72

15/15/45

NM_053685

HCN4

CGGTCACCATCATCTAGCCC/

TGCCATAAAGGATGGCCGTT

95/59.8/72

15/15/45

NM_021658

KCNQ1

GTGGTCCAACCTGCAACAAC/

CTTCATCAGGGCCTGTCTGG

95/59.0/72

15/15/45

NM_032073.1

KCNQ2

CGTGGTATTCGGTGTTGAGT/

CTCCGAAGTGCAGACGTAG

95/57.8/72

15/15/45

NM_133322.1

KCNQ3

TCATGTCCGTCAACCACGAG/

ACCGCTTTTCCCTCATCCAG

95/59.0/72

15/15/45

NM_031597

KCNQ4

AATCCGCATAAGCATCTCCCA/

GAAGCTCCAGCTTTTCTGCAC

95/59.0/72

15/15/45

AF249748.1

KCNQ5

AAGACGGGAGACAGTACGGA/

TTCCTGACTCTTGCGCGATT

95/58.0/72

15/15/45

NM_001134643

iNOS

GCTTGTCTCTGGGTCCTCTG/

CTCACTGGGACAGCACAGAA

95/59.0/72

15/15/45

NM_012611.3

CD206

AACAAGAATGGTGGGCAGTC/

CCTTTCAGTCCTTTGCAAGC

95/56.0/72

15/15/45

NM_001106123.2

Ki67

TCTTGGCACTCACAGTCCAG/

GCTGGAAGCAAGTGAAGTCC

95/58.0/72

15/15/45

NM_001271366.1

RPL13a

TCTCCGAAAGCGGATGAACA/

CAACACCTTGAGGCGTTCCA

 

15/15/45

NM_173340.2

Small interfering RNA HCN2

GCACCGGCAUUGUUAUUGAtt

UCAAUAACAAUGCCGGUGCga

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