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Fig. 5 | Journal of Neuroinflammation

Fig. 5

From: Transcriptomic analysis of brain tissues identifies a role for CCAAT enhancer binding protein β in HIV-associated neurocognitive disorder

Fig. 5

HIV Tat reduces neuronal and increases astroglial C/EBPβ mRNA and protein in vitro. Neuronal and astroglial cells were treated with recombinant HIV proteins (gp120, nef or Tat) for one or four days. a Relative expression of mRNA for C/EBPβ in neurons exposed to recombinant HIV proteins compared to vehicle-treated neurons. b Immunoblot for C/EBPβ using nuclear-enriched lysates from neurons exposed to vehicle or recombinant HIV proteins. c Quantification of densitometry of the the ~ 42 kDa band corresponding to C/EBPβ protein. d Relative expression of mRNA for C/EBPβ in astrocytes exposed recombinant HIV proteins compared to vehicle-treated astrocytes. e Immunoblot for C/EBPβ using nuclear-enriched lysates from astrocytes exposed to vehicle or recombinant HIV proteins. f Quantification of densitometry of the ~ 42 kDa band corresponding to C/EBPβ protein. Statistical analyses were performed by two-way ANOVA followed by Bonferonni’s post-hoc test

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