Fig. 1

Microglial densitometric changes observed in the striatum over the course of HD pathology. Picture examples showing microglial density and distribution in wild-type (WT) mice at 3 weeks (a), 10 weeks (b), and 13 weeks (c) of age and R6/2 (HTT) mice at 3 weeks (d), 10 weeks (e), and 13 weeks (f) of age, as identified using anti-IBA1 staining, captured using a brightfield microscope. Scale bar = 10 μm. Quantitative analysis of microglial density (g), nearest neighbor distance (h), and spacing index (i) performed at all three ages in both WT and HTT conditions. N = 4 animals per condition. Asterisk denotes the difference from WT, blue number sign denotes the difference between ages in WT mice, and red number sign denotes the difference between ages in R6/2 mice; *^,#p < 0.05, **^,##p < 0.01, ^###p < 0.001