Fig. 1

Effects of JWH-133 pretreatment on hindlimb motor function and BSCB permeability after I/R injury. a Neurological scores investigated by BBB Locomotor Scale at each time point during the 7 days observation after injury in each group. b Percentage of water content in the spinal cord tissue. c Amount of Evans blue (EB) in the spinal cord tissue. d Red fluorescence of EB extravasation in the spinal cord in the sham and I/R groups along the entire time course from 1 to 7 days after injury. Quantification of EB is represented as the average integrated optical density (IOD). Almost no red fluorescence displayed in the spinal cord of the sham group. I/R injury increased EB extravasation (red) at each observed time point, especially in the gray matter, with the maximal level at day 2 after injury. e Red fluorescence of EB extravasation in the spinal cord in each group at day 2 after injury. Quantification of EB is represented as the average IOD. In comparison to I/R group, EB extravasation was obviously lower in JWH or TAK group, especially in the gray matter. AM630 pretreatment diminished the effects of JWH-133. All results are represented as mean ± SEM (n = 5 per group at each time point). *P < 0.05 vs. sham group; #P < 0.05 vs. I/R group; & P < 0.05 vs. day 1; $ P < 0.05 vs. day 2. Scale bars are 200 μm