Fig. 2

Effects of JWH-133 pretreatment on the protein expression of ZO-1, TLR4, and MMP9 after I/R injury in the spinal cord tissue. a Protein expression of CB2R, ZO-1, TLR4, and MMP9 in the sham and I/R groups along the entire time course from 1 to 7 days after injury. I/R injury resulted in downregulation of ZO-1 and upregulation of TLR4/MMP9 during 7 days after injury, and this effect was most obvious at day 2 after injury. The expression of CB2R following I/R injury showed a sustained high level from day 1 to day 7 after injury. b Protein expression of ZO-1, TLR4, and MMP9 in each group at day 2 after injury. JWH-133 or TAK-242 treatment increased the ZO-1 protein level and reduced the level of TLR4/MMP9 comparing with I/R group. All results are represented as mean ± SEM (n = 5 per group at each time point). *P < 0.05 vs. sham group; #P < 0.05 vs; & P < 0.05 vs. day 1; $ P < 0.05 vs. day 2. I/R group