Fig. 1From: Normalization of magnesium deficiency attenuated mechanical allodynia, depressive-like behaviors, and memory deficits associated with cyclophosphamide-induced cystitis by inhibiting TNF-α/NF-κB signaling in female ratsFlowchart of the experimental procedure. a The study consisted of three parts. In part I, western blot analyses were performed at the three time points, day 8, day 12, and day 20, to determine the expression level of TNF-α/NF-κB, IL-1β, and NR2B in CYP-induced cystitis animals. The concentration of Mg2+ was also measured at the three time points. In part II, after treatment with L-TAMS, behavioral tests and molecular experiments were performed to investigate the effect of L-TAMS in the CYP-treated animals. The details in part II were shown in b. Additionally, in part III, the effect of inhibiting NF-κB signaling was determined by behavioral testsBack to article page