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Fig. 5 | Journal of Neuroinflammation

Fig. 5

From: NK cell-derived exosomes carry miR-207 and alleviate depression-like symptoms in mice

Fig. 5

MiR-207 played an important role in astrocyte activation through Tril. a The relative expression of miR-207 in exosomes extracted from NK cells of unstressed mice compared with exosomes extracted from NK cells of stressed mice (**P < 0.01 vs. exo (stress) group; n = 6). b Relative luciferase activity of Tril in the presence of the indicated treatments. Prediction of binding sites between miRNA and Tril and the effects of Tril mutant and wild-type vectors in the presence of miR-207 on fluorescence signal intensity (***P < 0.001 compared with miR-207 and m-TRIL-3UTR-wt group; n = 3). (C) qPCR analysis of Tril mRNA expression after transfection of miR-207 mimic in astrocytes (**P < 0.01 compared with the mimic NC group; n = 6). d Western blot analysis of the protein expression of NF-κB p65, p-p65, IKKα, and GAPDH (*P < 0.05 compared with the mimic NC group; n = 3). e ELISA analysis of IL-1β concentration after transfection of miR-207 mimic into astrocytes (**P < 0.01 compared with the mimic NC group; n = 6). f ELISA analysis of IL-6 concentration after transfection of miR-207 mimic into astrocytes (**P < 0.01 compared with the mimic NC group; n = 6). g ELISA analysis of TNF-α concentration after transfection of miR-207 mimic into astrocytes (*P < 0.05 compared with the mimic NC group; n = 6)

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