Skip to main content
Fig. 6 | Journal of Neuroinflammation

Fig. 6

From: NK cell-derived exosomes carry miR-207 and alleviate depression-like symptoms in mice

Fig. 6

In vitro and vivo antidepressant activity of an exo (miR-207 inhibitor). ac The in vitro antidepressant activity of exo (miR-207 inhibitor), transfection of which decreased miR-207 levels in exosomes (*P < 0.05, **P < 0.01 compared with the exo (inhibitor NC) group; n = 6). a NK cells were transfected with miR-207 inhibitor. After a 48-h incubation, transfected NK cell culture medium was collected for exosome extraction. MiR-207 levels in exosomes were measured and found to be decreased compared with those of the exosomes extracted from NK cells transfected with miR-207 inhibitor NC. b Expression of astrocyte Tril after incubation with exosomes with low miR-207 expression were detected by qPCR. c Expression of inflammatory factors (IL-1β, IL-6, and TNF-α) by astrocytes after they were incubated with exosomes with low miR-207 expression. dh Antidepressant activity of exo (miR-207 inhibitor), which resulted in lower miR-207 levels in exosomes. The animal grouping strategy is shown in Table 2 (*P < 0.05, **P < 0.01, and ***P < 0.001 compared with the stress group; n = 5 mice per group). d Behavioral test (OFT), which included horizontal movements (defined as at least three paws in a square), vertical movements (defined as at least three paws in a square), and exercise time. e Behavioral test (FST) (recorded immobile time in seconds). f Behavioral test (TST) (immobility was defined as hanging in a downright direction with only small movements). g The concentrations of inflammatory factors, including IL-6, IL-1β, and TNF-α, in mouse brain was measured by ELISA. h The concentrations of monoamine factors, 5-HT, DA, and NE, in mouse brain was measured by ELISA

Back to article page