Table 4 Sequences of the primers
From: NK cell-derived exosomes carry miR-207 and alleviate depression-like symptoms in mice
Type | Sequence (5′ to 3′) | Manufacturer |
|---|---|---|
miR-207 primer | F 5′-TTTGCTTCTCCTGGCTCTCC-3′ R 5′-TATCCTTGTTCACGAGTCCTTCAC-3′ | GenePharma, Shanghai, China |
Tril primer | F 5′-CTATGTATGCCGTTGGGGTAGG-3′ F 5′-AGCTTTTCACTTATTTCGCCCAT-3′ | General Biosystems, Anhui, China |
GAPDH primer | F 5′-CACTGAGCATCTCCCTCACA-3′ R 5′-GATTGAGCCTGCTTCACCTC-3′ | General Biosystems, Anhui, China |
miR-207 mimic/agomir | Sense 5′-GCUUCUCCUGGCUCUCCUCCCUC-3′ Antisense 5′-GGGAGGAGAGCCAGGAGAAGCUU-3′ | GenePharma, Shanghai, China |
miR-207 mimic NC | Sense 5′-UUCUCCGAACGUGUCACGUTT-3′ Antisense 5′-ACGUGACACGUUCGGAGAATT-3′ | GenePharma, Shanghai, China |
miR-207 inhibitor | 5′-GAGGGAGGAGAGCCAGGAGAAGC-3′ | ABM, USA |
miR-207 inhibitor NC | 5′-CAGUACUUUUGUGUAGUACAA-3′ | ABM, USA |
U6 | F 5′-CAGCACATATACTAAAATTGGAACG-3′ R 5′-ACGAATTTGCGTGTCATCC-3′ | GenePharma, Shanghai, China |