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Fig. 2 | Journal of Neuroinflammation

Fig. 2

From: Independent and inter-dependent immunoregulatory effects of NCF1 and NOS2 in experimental autoimmune encephalomyelitis

Fig. 2

The enhanced EAE is not associated with cytokine productions of T cell subsets. a NOS2-deficient mice were immunized with the MOG79-96 peptides and sacrificed at day 10 after immunization. The EAE severity was higher in double-deficient mice (Ncf1*/*.Nos2−/− genotype) than that of their littermate controls (Ncf1+/+.Nos2−/− genotype), of which inguinal lymph node cells were analyzed by using flow cytometric analysis as follows. b Representative plots for IFN-γ and IL-17-positive cells in CD4+ T cells with or without PMA and ionomycin stimulation. c The frequencies of IFN-γ and IL-17-positive cells in CD4+ T cells were similar after stimulation with PMA and ionomycin for 4 h. d Concentrations of IFN-γ and IL-17 in lymph node cell culture supernatant after re-stimulation ex vivo using MOG79-96 peptides for 96 h. The number of mice in each group is 8. ***p < 0.001 as determined by the Mann-Whitney U test

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