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Fig. 3 | Journal of Neuroinflammation

Fig. 3

From: Independent and inter-dependent immunoregulatory effects of NCF1 and NOS2 in experimental autoimmune encephalomyelitis

Fig. 3

NCF1 deficiency reduces IL-1β release from neutrophils in spleens prior to clinical onset. a Representative flow cytometry plot for neutrophils in the spleen. The solenocytes were collected from NCF1-deficient (Ncf1*/*.Nos2+/+ genotype) and NCF1-sufficient mice (Ncf1+/+.Nos2+/+ genotype) at day 4 post immunization. The frequency and cell number of neutrophils in the spleen are shown, upon stimulation with PMA. b Mean florescence intensities (MFIs) of DHR and DCF staining in neutrophils are shown, after cells were incubated ex vivo with PMA, PMA and ionomycin, or DMSO as the control. c MFIs of NCF1 and NOS2 staining in neutrophils are shown. d MFIs of IL-1β and TNF-α staining in neutrophils are shown. The number of mice is 7 per group. **p < 0.01 and ***p < 0.001 as determined by the Mann-Whitney U test

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