Fig. 4

Double deficiencies of NCF1 and NOS2 increase IL-1β release from neutrophils in the spleen prior to clinical onset. a Representative flow cytometry plots for neutrophils in the spleen. The splenocytes were collected from double-deficient mice (Ncf1^*/*.Nos2^−/− genotype) and their littermates NOS2-deficient mice (Ncf1^+/+.Nos2^−/− genotype), as well as wild-type mice (Ncf1^+/+.Nos2^+/+ genotype) at day 4 post immunization. b The frequency and cell number of neutrophils in the spleen are shown, upon stimulation with PMA. c The MFIs of IL-1β staining in splenic neutrophils are shown. d The cytokine levels of spleen cell culture supernatants were determined after incubation with MOG_79-96 peptides for 24 h. The number of mice per group is 6. *p < 0.05 and **p < 0.01 as determined by the Mann-Whitney U test