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Fig. 1. | Journal of Neuroinflammation

Fig. 1.

From: Mitigation of helium irradiation-induced brain injury by microglia depletion

Fig. 1.

Schematic of the experimental design. 6-months old adult male mice (C57Bl6/J and Thy1-EFGP+) received whole body exposure to the 4He particles (400 MeV/n, 30 cGy at the Brookhaven National Laboratory). Control (0 Gy) and irradiated (30 cGy) mice received either control chow or PLX5622 chow (1200 PPM) beginning from two-week post-irradiation and continued throughout the study. Mice were administered hippocampal- and frontal cortex-dependent cognitive testing four-week post-irradiation including novel object recognition (NOR), novel place recognition (NPR), object in place (OiP), temporal order (TO), elevated plus maze (EPM), forced swim test (FST) and, in the end, fear extinction memory (FE) tasks. A separate cohort of mice was used for the electrophysiology measurements. After completion of behavior testing, mice were euthanized and tissues were collected for the immunohistochemistry and neuron structure analyses

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