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Fig. 4 | Journal of Neuroinflammation

Fig. 4

From: Mitigation of helium irradiation-induced brain injury by microglia depletion

Fig. 4

Treatment with PLX5622 depletes microglia from the control and 4He-irradiated brains. a, b Immunofluorescence staining, confocal microscopy and 3D algorithm-based volumetric quantification of pan microglial marker IBA-1 (green; nuclear counter stain, DAPI, blue) show > 90% depletion of microglia in the control (0 Gy + PLX5622) and 4He-irradiated (30 cGy + PLX5622) hippocampus at 8-week post-PLX5622 treatments (***p < 0.001 compared to 0 Gy + Con chow and 30 cGy + Con chow). 4He irradiation did not alter the IBA-1 immunoreactivity (0 Gy + Con chow vs. 30 cGy + PLX5622). c, d Exposure to 4He particles elevated microglial activation maker, CD68, immunoreactivity (***p < 0.001 compared to 0 Gy + Con chow). Treatment with PLX5622 for 8 weeks eliminates 80–90% of CD68+ activated microglia (red; nuclear counter stain, DAPI, blue) in the control (0 Gy + PLX5622) and 4He-irradiated (30 cGy + PLX5622) hippocampus (**p < 0.001 compared to 0 Gy + Con chow and 30 cGy + Con chow). Data are presented as mean ± SEM (n = 4–6/group). p values are derived from two-way ANOVA and Bonferroni’s multiple comparisons test. Scale bar: 50 μm, a 100 μm, b 5 μm, inserts, a, b

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