Fig. 6
From: Mitigation of helium irradiation-induced brain injury by microglia depletion
Effect of 4He irradiation or PLX5622 treatment on astrogliosis. a–f Immunofluorescence staining, confocal microscopy, and 3D algorithm-based volumetric quantification of astrocytic marker GFAP (green; nuclear counter stain, DAPI, blue) show increased gross GFAP immunoreactivity in the control mice receiving PLX5622 (0 Gy + PLX5622) at 8 weeks post-PLX5622 treatments (*p < 0.001 compared to 0 Gy + Con chow and 30 cGy + Con chow). 4He-irradiated mice receiving PLX5622 showed decreased GFAP immunoreactivity (*p < 0.001, 30 cGy + PLX5622 compared to all other groups). a1–d1 Exposure to 4He particles and PLX5622 treatment reduced astrogliosis (GFAP volume per cell; *p < 0.001 compared to all other groups). Data are presented as mean ± SEM (n = 4/group, e; and n = 21–24 GFAP+ cells/group, f). p values are derived from two-way ANOVA and Bonferroni’s multiple comparisons test. Scale bars: 30 μm, a–d; 5 μm, a1–d1