Fig. 2
From: Glioblastoma hijacks microglial gene expression to support tumor growth
RNA Expression changes are most pronounced in EV-GFPpos microglia compared to GFPneg microglia. a In unsupervised clustering of the top 750 most variable genes, microglia cluster together based on tumor status and GFP uptake status. b Comparative analysis of differential expression levels of EV-GFPpos microglia and GFPneg microglia compared to control microglia showed 380 shared significantly upregulated genes (green). Overall, the differential expression was higher for EV-GFPpos microglia. These microglia expressed 1426 significantly upregulated and 1196 downregulated genes (red). c Most genes significantly changed between EV-GFPpos microglia and GFPneg microglia and were also significantly altered in EV-GFPpos microglia compared to control. d These patterns were confirmed in the comparisons of GFPneg to either GFPpos or control. e Venn diagram showed overlap between top 750 expressed genes. GFPneg tumor microglia shared most genes with control microglia and EV-GFPpos microglia. This confirmed that EV-GFPpos microglia represent the most altered tumor-associated phenotype