Table 1 Primers used in qPCR analysis
Primer | Sequence |
|---|---|
Reference genes | |
β-Actin | FW: TTTGAGACCTTCAACACCCC |
RV: ATAGCTCTTCTCCAGGGAGG | |
GAPDH | FW: ACATCAAATGGGGTGATGCT |
RV: GTGGTTCACACCCATCACAA | |
18S rRNA | FW: ATGGTAGTCGCCGTGCCTA |
RV: CTGCTGCCTTCCTTGGATG | |
YWHAZ | FW: TTGAGCAGAAGACGGAAGGT |
RV: GAAGCATTGGGGATCAAGAA | |
Target genes | |
GR | RT2 qPCR Primer Assay (PPR52805B, Qiagen) |
MR | FW: GGCAGCTGCAAAGTCTTCTT |
RV: GACAGTTCTTTCGCCGAATC | |
NFκB | RT2 qPCR Primer Assay (PPR42746A, Qiagen) |
IκBα | FW: CAGGATTCTGCAGGTCCACT |
RV: TGGAGCACTTGGTGACTTTG | |
IL6 | RT2 qPCR Primer Assay (PPR06483B, Qiagen) |
IL10 | RT2 qPCR Primer Assay (PPR06479A, Qiagen) |
CD11B | FW: GAAGCCTTGGCGTGTGATAG |
RV: GAGCAGTTTGTTCCCAAGGG | |
MKP1 | FW: GCTCCACTCAAGTCTTCTTCCTCCAA |
RV: TGGACTGTTTGCTGCACAGCTCAG | |
IGF1 | FW: GCTCTTCAGTTCGTGTGTGG |
RV: TGAGTCTTGGGCATGTCAGT | |