Fig. 3
Patch-clamp evaluation on the dose curve of Zinc20267861. Dose curve of 0.5 μM–5 mM of Zinc20267861 with BayK8644 2 μM and Amlodipine 2 μM used as control in smooth muscle cells (SMCs). Representative patch-clamp records (400 ms) of Ca2+ current I–V curves (a). Ca2+ current amplitude picoampere (pA) vs. stimuli voltage (mV) with the channel peak opening is at 0 mV (b). Quantitative analysis of pA at channel peak opening of 0 mV (Basal, BayK8644 1 μM n = 8; Zinc20268761 2 μM, Amlodipine 2 μM n = 6) (c). One-way ANOVA test with Tukey multiple comparisons was used to determine statistical significance. n.s. p > 0.05; *p < 0.05; **p < 0.01; ***p < 0.001