Fig. 2

Induction of GPR120 expression in endothelial cells and macrophages of human IA lesions. The representative images of immunostaining of specimens from human IA lesion (the upper panels) and the control arterial wall (middle meningeal artery, the lower panels) for GPR120 (green), CD31, a marker for endothelial cells (red in a), CD68, a marker for macrophages (red in b) or smooth muscle α-actin (SMA), a marker for smooth muscle cells (red in c), nuclear staining by DAPI (blue) and merged images are shown. The magnified images corresponding to the squares in the left panels are also shown on a right. In d, the image of immunostaining without a primary antibody is shown as a negative control study. Bar; 10 μm. Note the induction of GPR120 in CD31-positive endothelial cells and CD68-positive macrophages while expression in smooth muscle cells is constitutive