Fig. 5

Inhibitory effect of EPA on the infiltration of macrophages in lesions. a, b Inhibitory effect of EPA on the infiltration of macrophages in lesions. Macrophages infiltrating in IA lesions from rats treated with vehicle or EPA (100 or 1000 mg/kg/day) for 12 days were visualized by immunostaining for CD68, a marker for macrophages. The representative images from immunohistochemistry for CD68 (green) or SMA, a marker for smooth muscle cells (red), nuclear staining by DAPI (blue) and merged images are shown (a). The magnified images corresponding to the squares are also shown in the lower panels (a). Bar; 10 μm. The number of infiltrating macrophages in IA lesions was calculated (vehicle, n = 8, 100 mg/kg/day, n = 10, 1000 mg/kg/day, n = 9). Data represents box-and-whisker plots (b). Statistical analysis was done by a Kruskal-Wallis test. *p < 0.05