Fig. 4

Representative flow cytometric plots showing different populations of dendric cells in the cornea of WT and rTg4510 mice at 9 months of age. a, b Gating strategies for enumeration of CD45⁺CD11c⁺ CD11b⁺ stromal DCs and CD45⁺CD11c⁺ CD11b⁻ epithelial DCs in WT and rTg4510 mice. c Frequencies of CD86⁺ stromal and epithelial DCs in two genotypes. rTg4510 mice had a higher proportion of CD86⁺ stromal DCs (48.8%) compared to WT (30.3%). Proportion of CD86⁺ epithelial DCs were also substantially higher in rTg4510 (4.74%) compared to WT (1.87%). d Proportion of CD80⁺ stromal DCs was 82.5% in WT and 88.% in rTg4510. Epithelial DCs, however, comprise a higher proportion of the CD80⁺ population in rTg4510 (6.94%) than WT (2.08%). e The corneas from rTg4510 mice had a higher percentage of CD86⁺ epithelial DCs and stromal DCs compared to WT as well as higher percentage of CD80⁺ epithelial DCs and stromal DCs at 9 months of age