Fig. 5

Spleen flow cytometric plots showing the phenotypes of CD45⁺ cell populations in WT and rTg4510 at 9 months. a Gating scheme for splenocytes isolation showing DAPI-population was used for the downstream analysis. b Total CD45⁺ cells were not different between WT (99.9%) and rTg4510 mice (99.8%). c The proportion of CD86⁺ and CD80⁺ population was low in WT and displayed no substantial changes in rTg4510 mice. d The absolute number of CD86⁺ and CD80⁺ showed no difference in the CD11c⁺ splenocytes between the two genotypes, but a higher number of CD86⁺ and CD80⁺ cells in the CD11b⁺ splenic immune subset of rTg4510 mice compared to WT mice